BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
MYH10
MCH-B, NMMHC-B, SMemb
myosin, heavy chain 10, non-muscle
GO Process (28)
GO Function (6)
GO Component (21)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- actin cytoskeleton organization [ISO]
- actin filament-based movement [ISO]
- actomyosin structure organization [ISO]
- adult heart development [ISO]
- axon guidance [ISO]
- axonogenesis [ISO]
- brain development [ISO]
- cardiac myofibril assembly [ISO]
- cell proliferation [ISO]
- cellular component movement [NAS]
- cerebellar Purkinje cell layer development [ISO]
- exocytosis [ISO]
- fourth ventricle development [ISO]
- in utero embryonic development [ISO]
- lateral ventricle development [ISO]
- mitotic cytokinesis [ISO]
- myofibril assembly [ISO]
- neuromuscular process controlling balance [ISO]
- neuron migration [ISO]
- neuron projection development [ISO]
- nuclear migration [ISO]
- plasma membrane repair [ISO]
- regulation of cell shape [ISO]
- retina development in camera-type eye [ISO]
- substrate-dependent cell migration, cell extension [ISO]
- third ventricle development [ISO]
- ventricular cardiac muscle cell development [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [ISO]
- actomyosin [ISO]
- axon [ISO]
- cell cortex [ISO]
- cleavage furrow [ISO]
- cytoplasm [ISO]
- dendritic spine [ISO]
- extracellular vesicular exosome [ISO]
- growth cone [ISO]
- midbody [ISO]
- mitochondrion [ISO]
- myosin II complex [ISO]
- myosin II filament [ISO]
- myosin complex [ISO]
- neuromuscular junction [ISO]
- neuron projection [ISO]
- neuronal cell body [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- spindle [ISO]
- stress fiber [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID