BAIT
PRKAB1
protein kinase, AMP-activated, beta 1 non-catalytic subunit
GO Process (4)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAP1A
RAP-1A, rap 1A
RAP1A, member of RAS oncogene family
GO Process (15)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- GTP catabolic process [ISO]
- Rap protein signal transduction [ISO, ISS]
- cellular response to cAMP [ISO, ISS]
- cellular response to nerve growth factor stimulus [IDA]
- establishment of endothelial barrier [ISO, ISS]
- negative regulation of synaptic vesicle exocytosis [ISO]
- nerve growth factor signaling pathway [IDA]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISO]
- positive regulation of Rap GTPase activity [IMP]
- positive regulation of neuron projection development [IMP, ISO]
- positive regulation of protein kinase activity [IMP]
- positive regulation of vasculogenesis [ISO, ISS]
- protein transport [ISO, ISS]
- regulation of cell junction assembly [ISO, ISS]
- small GTPase mediated signal transduction [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID