BAIT
PRKAB1
protein kinase, AMP-activated, beta 1 non-catalytic subunit
GO Process (4)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAB8A
Mel, Rab8
RAB8A, member RAS oncogene family
GO Process (17)
GO Function (5)
GO Component (23)
Gene Ontology Biological Process
- GTP catabolic process [IBA, ISO]
- Golgi vesicle fusion to target membrane [IBA, ISO]
- Rab protein signal transduction [IBA]
- axonogenesis [IMP]
- cellular response to insulin stimulus [IMP]
- cilium assembly [ISO, ISS]
- glucose homeostasis [IC]
- intracellular protein transport [IBA]
- positive regulation of glucose import [IC]
- protein localization to plasma membrane [IDA]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- regulation of long-term neuronal synaptic plasticity [IDA, IMP]
- regulation of protein localization [IMP]
- regulation of protein transport [IDA, IMP]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IBA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- centrosome [ISO]
- ciliary basal body [ISO]
- cilium [ISO, ISS]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- dendrite [IDA]
- endosome [IBA]
- extracellular vesicular exosome [ISO]
- neuronal cell body [IDA]
- nonmotile primary cilium [ISO]
- nucleolus [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- phagocytic vesicle [ISO, ISS]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA]
- primary cilium [ISO]
- recycling endosome [ISO]
- recycling endosome membrane [ISO, ISS]
- secretory granule membrane [IBA]
- synaptic vesicle [IBA]
- trans-Golgi network transport vesicle [IBA, ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID