BAIT
PRKAB1
protein kinase, AMP-activated, beta 1 non-catalytic subunit
GO Process (4)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ACTN4
actinin alpha 4
GO Process (8)
GO Function (6)
GO Component (15)
Gene Ontology Biological Process
- actin filament bundle assembly [ISO]
- negative regulation of cellular component movement [ISO]
- positive regulation of cellular component movement [ISO]
- positive regulation of pinocytosis [ISO]
- protein localization to tight junction [ISO]
- regulation of apoptotic process [IMP]
- response to hypoxia [IEP]
- tight junction assembly [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [ISO]
- cell-cell junction [ISO]
- cortical cytoskeleton [ISO]
- cytoplasm [IDA, ISO]
- cytosol [TAS]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- neuron projection [IDA]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [IDA, ISO]
- pseudopodium [ISO]
- ribonucleoprotein complex [ISO, ISS]
- stress fiber [IDA, ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID