BAIT
PRKAB1
protein kinase, AMP-activated, beta 1 non-catalytic subunit
GO Process (4)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAB7A
Rab7
RAB7A, member RAS oncogene family
GO Process (14)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- GTP catabolic process [IBA, ISO]
- Rab protein signal transduction [IBA]
- bone resorption [IMP]
- early endosome to late endosome transport [ISO, ISS]
- endosome to lysosome transport [IBA, ISO]
- epidermal growth factor catabolic process [ISO]
- intracellular protein transport [IBA]
- phagosome acidification [ISO, ISS]
- phagosome-lysosome fusion [IBA, ISO, ISS]
- positive regulation of exosomal secretion [ISO]
- protein targeting to lysosome [ISO]
- protein to membrane docking [ISO]
- regulation of autophagic vacuole assembly [ISO]
- retrograde transport, endosome to Golgi [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- alveolar lamellar body [IDA]
- cytoplasm [ISO]
- cytoplasmic vesicle [IDA]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- late endosome [IDA, ISO, TAS]
- lysosome [IBA, ISO]
- phagocytic vesicle [IBA, ISO, ISS]
- pre-autophagosomal structure membrane [ISO]
- retromer complex [ISO]
- terminal bouton [IDA]
- vacuolar membrane [IBA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID