BAIT
PRKAB1
protein kinase, AMP-activated, beta 1 non-catalytic subunit
GO Process (4)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CLTC
clathrin, heavy chain (Hc)
GO Process (8)
GO Function (8)
GO Component (18)
Gene Ontology Biological Process
- Golgi organization [IMP]
- mitotic nuclear division [IMP, ISO]
- negative regulation of hyaluronan biosynthetic process [ISO, ISS]
- negative regulation of protein localization to plasma membrane [ISO]
- osteoblast differentiation [ISO]
- receptor internalization [ISO]
- receptor-mediated endocytosis [IMP, ISO]
- transferrin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- clathrin coat [IDA, TAS]
- clathrin complex [ISO]
- clathrin-coated endocytic vesicle membrane [TAS]
- clathrin-coated vesicle [ISO]
- cytoplasm [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- membrane coat [ISO]
- mitochondrion [ISO]
- myelin sheath [ISO]
- protein complex [ISO]
- sarcolemma [IDA]
- spindle [IDA, ISO]
- terminal bouton [IDA]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PRKAB1 CLTC | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | High | - | BioGRID | 2546432 |
Curated By
- BioGRID