BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RHOA
Arha, Arha2
ras homolog family member A
GO Process (57)
GO Function (7)
GO Component (13)
Gene Ontology Biological Process
- GTP catabolic process [IDA, ISO]
- actin cytoskeleton organization [ISO, TAS]
- androgen receptor signaling pathway [ISO]
- apical junction assembly [ISO, ISS]
- apolipoprotein A-I-mediated signaling pathway [ISO]
- cell adhesion [ISO]
- cell differentiation [ISO]
- cell morphogenesis [ISO]
- cell-matrix adhesion [ISO]
- cellular response to progesterone stimulus [NAS]
- cerebral cortex cell migration [ISO]
- cleavage furrow formation [ISO, ISS]
- cytoskeleton organization [ISO]
- forebrain radial glial cell differentiation [ISO]
- negative chemotaxis [ISO]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- negative regulation of cell death [IMP]
- negative regulation of intracellular steroid hormone receptor signaling pathway [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of neuron differentiation [IMP]
- neuron projection morphogenesis [IEP]
- ossification involved in bone maturation [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO, ISS]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of cell adhesion [IEP]
- positive regulation of cell growth [IEP, IMP]
- positive regulation of cell migration [IEP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of cytokinesis [ISO, ISS]
- positive regulation of neuron apoptotic process [IMP]
- positive regulation of neuron differentiation [ISO]
- positive regulation of podosome assembly [ISO]
- positive regulation of smooth muscle contraction [IMP]
- positive regulation of stress fiber assembly [ISO]
- positive regulation of translation [IEP]
- positive regulation of vasoconstriction [IEP]
- regulation of actin polymerization or depolymerization [IEP]
- regulation of calcium ion transport [IMP]
- regulation of cell migration [ISO, ISS]
- regulation of dendrite development [IMP]
- regulation of neural precursor cell proliferation [ISO]
- regulation of neuron projection development [ISO]
- regulation of osteoblast proliferation [ISO]
- regulation of transcription from RNA polymerase II promoter [ISO]
- response to amino acid [IEP]
- response to drug [IDA, IEP]
- response to ethanol [IEP]
- response to glucocorticoid [IEP]
- response to glucose [IEP]
- response to hypoxia [IEP]
- response to mechanical stimulus [IEP]
- skeletal muscle tissue development [ISO]
- spindle assembly involved in mitosis [ISO]
- stress fiber assembly [ISO]
- stress-activated protein kinase signaling cascade [IMP]
- substantia nigra development [ISO]
- trabecula morphogenesis [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKAA1 RHOA | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID