BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAC1
ras-related C3 botulinum toxin substrate 1
GO Process (52)
GO Function (10)
GO Component (16)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO]
- GTP catabolic process [IDA, ISO]
- Rac protein signal transduction [TAS]
- Wnt signaling pathway, planar cell polarity pathway [ISO]
- actin cytoskeleton organization [IDA, ISO]
- actin filament organization [IMP]
- actin filament polymerization [ISO]
- anatomical structure arrangement [ISO]
- auditory receptor cell morphogenesis [ISO]
- axon guidance [ISO]
- bone resorption [IDA]
- cell adhesion [ISO]
- cell migration [ISO]
- cell motility [ISO, ISS]
- cell proliferation [IMP]
- cell-cell junction organization [ISO]
- cellular component movement [IMP]
- cerebral cortex radially oriented cell migration [ISO]
- chemotaxis [IMP]
- cochlea morphogenesis [ISO]
- cytoskeleton organization [ISO]
- dendrite development [ISO]
- dendrite morphogenesis [ISO]
- dopaminergic neuron differentiation [ISO]
- embryonic olfactory bulb interneuron precursor migration [ISO]
- endocytosis [ISO]
- engulfment of apoptotic cell [ISO]
- epithelial cell morphogenesis [ISO]
- hyperosmotic response [ISO]
- lamellipodium assembly [ISO]
- localization within membrane [ISO]
- mast cell chemotaxis [IMP]
- negative regulation of interleukin-23 production [ISO]
- phagocytosis, engulfment [ISO]
- positive regulation of DNA replication [IMP]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of cell-substrate adhesion [ISO]
- positive regulation of focal adhesion assembly [ISO]
- positive regulation of lamellipodium assembly [ISO]
- positive regulation of neutrophil chemotaxis [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [ISO]
- positive regulation of protein phosphorylation [ISO, ISS]
- positive regulation of stress fiber assembly [ISO]
- positive regulation of substrate adhesion-dependent cell spreading [ISO]
- protein localization to plasma membrane [ISO]
- regulation of cell migration [ISO, ISS]
- regulation of respiratory burst [ISO]
- ruffle assembly [ISO]
- ruffle organization [ISO]
- semaphorin-plexin signaling pathway [IMP]
- small GTPase mediated signal transduction [ISO]
- substrate adhesion-dependent cell spreading [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [IDA]
- actin filament [ISO]
- cell projection [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic membrane-bounded vesicle [ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytoplasmic vesicle [IDA]
- cytosol [IDA]
- extracellular vesicular exosome [ISO]
- extrinsic component of plasma membrane [ISO]
- focal adhesion [ISO]
- lamellipodium [ISO]
- membrane [IDA, ISO]
- phagocytic cup [ISO]
- ruffle membrane [ISO]
- trans-Golgi network [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKAA1 RAC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID