An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity.

Sandi MJ, Marshall CB, Balan M, Coyaud E, Zhou M, Monson DM, Ishiyama N, Chandrakumar AA, La Rose J, Couzens AL, Gingras AC, Raught B, Xu W, Ikura M, Morrison DK, Rottapel R

The PAR-1-MARK pathway controls cell polarity through the phosphorylation of microtubule-associated proteins. Rho-Rac guanine nucleotide exchange factor 2 (ARHGEF2), which activates Ras homolog family member A (RHOA), is anchored to the microtubule network and sequestered in an inhibited state through binding to dynein light chain Tctex-1 type 1 (DYNLT1). We showed in mammalian cells that liver kinase B1 (LKB1) activated ... [more]

Sci Signal Oct. 31, 2017; 10(503); [Pubmed: 29089450]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CEP170 ARHGEF2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Gupta GD (2015)	High	-	BioGRID	1431124

Curated By

BioGRID

Interaction Summary

ARHGEF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rac GTPase binding [IDA]Rac guanyl-nucleotide exchange factor activity [IDA]Rho GTPase binding [IDA]Rho guanyl-nucleotide exchange factor activity [IDA]microtubule binding [IDA]protein binding [IPI]transcription factor binding [ISS]zinc ion binding [NAS]

Gene Ontology Cellular Component

CEP170