BAIT
FLT3
CD135, FLK-2, FLK2, STK1, RP11-153M24.3
fms-related tyrosine kinase 3
GO Process (21)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
- B cell differentiation [ISS]
- cellular response to cytokine stimulus [ISS, TAS]
- common myeloid progenitor cell proliferation [ISS]
- cytokine-mediated signaling pathway [ISS]
- dendritic cell differentiation [ISS]
- hemopoiesis [IDA]
- leukocyte homeostasis [ISS]
- lymphocyte proliferation [ISS]
- myeloid progenitor cell differentiation [ISS]
- peptidyl-tyrosine phosphorylation [TAS]
- positive regulation of MAP kinase activity [TAS]
- positive regulation of MAPK cascade [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of phosphatidylinositol 3-kinase activity [TAS]
- positive regulation of phosphatidylinositol 3-kinase signaling [TAS]
- positive regulation of tyrosine phosphorylation of STAT protein [TAS]
- pro-B cell differentiation [ISS]
- protein autophosphorylation [TAS]
- regulation of apoptotic process [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
- vascular endothelial growth factor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GSK3A
glycogen synthase kinase 3 alpha
GO Process (33)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cardiac left ventricle morphogenesis [ISS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [IMP]
- cellular response to interleukin-3 [ISS]
- endoplasmic reticulum unfolded protein response [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- extrinsic apoptotic signaling pathway in absence of ligand [ISS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [ISS]
- negative regulation of TOR signaling [ISS]
- negative regulation of UDP-glucose catabolic process [IC]
- negative regulation of canonical Wnt signaling pathway [TAS]
- negative regulation of cell growth involved in cardiac muscle cell development [ISS]
- negative regulation of glucose import [IMP]
- negative regulation of glycogen (starch) synthase activity [TAS]
- negative regulation of glycogen biosynthetic process [TAS]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of transferase activity [IMP]
- negative regulation of type B pancreatic cell development [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of adrenergic receptor signaling pathway [ISS]
- positive regulation of cAMP biosynthetic process [ISS]
- positive regulation of glycogen (starch) synthase activity [ISS]
- positive regulation of heart contraction [ISS]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [ISS]
- positive regulation of protein catabolic process [NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISS]
- protein phosphorylation [IDA]
- regulation of systemic arterial blood pressure [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Genome-Wide CRISPR Screen Identifies Genes Critical for Resistance to FLT3 Inhibitor AC220.
Acute myeloid leukemia (AML) is a malignant hematopoietic disease and the most common type of acute leukemia in adults. The mechanisms underlying drug resistance in AML are poorly understood. Activating mutations in FMS-like tyrosine kinase 3 (FLT3) are the most common molecular abnormality in AML. Quizartinib (AC220) is a potent and selective second-generation inhibitor of FLT3. It is in clinical ... [more]
Cancer Res. Dec. 15, 2016; 77(16);4402-4413 [Pubmed: 28625976]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: MV4-11 cells
- Experimental Setup:Drug Exposure, 3 nM AC220
- GIST: A-phenotypic positive genetic interaction
- Library: GECKO
- Significance Threshold: Only screen hits were published
Curated By
- BioGRID