BAIT

OCIAD1

ASRIJ, OCIA, TPA018
OCIA domain containing 1
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

UQCRC2

MC3DN5, QCR2, UQCR2
ubiquinol-cytochrome c reductase core protein II
GO Process (5)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

OCIAD1 Controls Electron Transport Chain Complex I Activity to Regulate Energy Metabolism in Human Pluripotent Stem Cells.

Shetty DK, Kalamkar KP, Inamdar MS

Pluripotent stem cells (PSCs) derive energy predominantly from glycolysis and not the energy-efficient oxidative phosphorylation (OXPHOS). Differentiation is initiated with energy metabolic shift from glycolysis to OXPHOS. We investigated the role of mitochondrial energy metabolism in human PSCs using molecular, biochemical, genetic, and pharmacological approaches. We show that the carcinoma protein OCIAD1 interacts with and regulates mitochondrial complex I activity. ... [more]

Stem Cell Reports Jul. 10, 2018; 11(1);128-141 [Pubmed: 29937147]

Throughput

  • Low Throughput

Additional Notes

  • Immunoprecipitation of native/endogenous OCIAD1 in human embryonic stem cells (hESCs)

Curated By

  • BioGRID