Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

An ear-core interaction regulates the recruitment of the AP-3 complex to membranes.

Lefrancois S, Janvier K, Boehm M, Ooi CE, Bonifacino JS

AP-3 is a heterotetrameric adaptor involved in the biogenesis of lysosome-related organelles. The function of AP-3 as an adaptor relies on its ability to bind to membranes in an Arf-dependent fashion and to recognize sorting signals in the cytosolic tails of the transmembrane cargo. Here, we report an interdomain interaction involving the ear domain of the delta subunit and the ... [more]

Dev. Cell Oct. 01, 2004; 7(4);619-25 [Pubmed: 15469849]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AP3S2 AP3D1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Dell'Angelica EC (1997)	Low	-	BioGRID	-
AP3D1 AP3S2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lefrancois S (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AP3D1

Gene Ontology Biological Process

Gene Ontology Molecular Function

transporter activity [TAS]

Gene Ontology Cellular Component

AP3S2