An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

An ear-core interaction regulates the recruitment of the AP-3 complex to membranes.

Lefrancois S, Janvier K, Boehm M, Ooi CE, Bonifacino JS

AP-3 is a heterotetrameric adaptor involved in the biogenesis of lysosome-related organelles. The function of AP-3 as an adaptor relies on its ability to bind to membranes in an Arf-dependent fashion and to recognize sorting signals in the cytosolic tails of the transmembrane cargo. Here, we report an interdomain interaction involving the ear domain of the delta subunit and the ... [more]

Dev. Cell Oct. 01, 2004; 7(4);619-25 [Pubmed: 15469849]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SCARB2

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

AP3S2