BAIT

MAK5

L000000979, YBR142W
Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
Saccharomyces cerevisiae (S288c)
PREY

NOP8

L000004849, YOL144W
Nucleolar protein required for 60S ribosomal subunit biogenesis
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

RNA helicases mediate structural transitions and compositional changes in pre-ribosomal complexes.

Bruening L, Hackert P, Martin R, Davila Gallesio J, Aquino GRR, Urlaub H, Sloan KE, Bohnsack MT

Production of eukaryotic ribosomal subunits is a highly dynamic process; pre-ribosomes undergo numerous structural rearrangements that establish the architecture present in mature complexes and serve as key checkpoints, ensuring the fidelity of ribosome assembly. Using in vivo crosslinking, we here identify the pre-ribosomal binding sites of three RNA helicases. Our data support roles for Has1 in triggering release of the ... [more]

Nat Commun Dec. 19, 2017; 9(1);5383 [Pubmed: 30568249]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NOP8 MAK5
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1597BioGRID
1951710

Curated By

  • BioGRID