BAIT
KDR
CD309, FLK1, VEGFR, VEGFR2
kinase insert domain receptor
GO Process (31)
GO Function (9)
GO Component (9)
Gene Ontology Biological Process
- angiogenesis [TAS]
- calcium-mediated signaling using intracellular calcium source [IMP]
- cell migration involved in sprouting angiogenesis [ISS]
- cellular response to vascular endothelial growth factor stimulus [IDA, IMP]
- embryonic hemopoiesis [ISS]
- endothelium development [ISS]
- extracellular matrix organization [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of endothelial cell apoptotic process [IDA]
- peptidyl-tyrosine autophosphorylation [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of MAPK cascade [IDA]
- positive regulation of angiogenesis [IMP]
- positive regulation of cell migration [IDA, IMP]
- positive regulation of cell proliferation [IDA, IMP]
- positive regulation of endothelial cell migration [IMP]
- positive regulation of endothelial cell proliferation [IMP]
- positive regulation of focal adhesion assembly [IDA]
- positive regulation of nitric-oxide synthase biosynthetic process [IDA, IMP]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of positive chemotaxis [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of vasculogenesis [ISS]
- protein autophosphorylation [IDA]
- regulation of cell shape [IDA]
- signal transduction by phosphorylation [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
- vascular endothelial growth factor receptor signaling pathway [IDA, IMP, TAS]
- vascular endothelial growth factor signaling pathway [IDA]
- vasculogenesis [ISS]
Gene Ontology Molecular Function- Hsp90 protein binding [TAS]
- growth factor binding [IPI]
- integrin binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA]
- receptor signaling protein tyrosine kinase activity [TAS]
- transmembrane receptor protein tyrosine kinase activity [TAS]
- vascular endothelial growth factor binding [IPI]
- vascular endothelial growth factor-activated receptor activity [IDA]
- Hsp90 protein binding [TAS]
- growth factor binding [IPI]
- integrin binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA]
- receptor signaling protein tyrosine kinase activity [TAS]
- transmembrane receptor protein tyrosine kinase activity [TAS]
- vascular endothelial growth factor binding [IPI]
- vascular endothelial growth factor-activated receptor activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
UBC
HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- DNA repair [TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- I-kappaB kinase/NF-kappaB signaling [TAS]
- JNK cascade [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- Notch receptor processing [TAS]
- Notch signaling pathway [TAS]
- RNA metabolic process [TAS]
- T cell receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- carbohydrate metabolic process [TAS]
- cellular response to hypoxia [TAS]
- cytokine-mediated signaling pathway [TAS]
- endosomal transport [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- glucose metabolic process [TAS]
- glycogen biosynthetic process [TAS]
- innate immune response [TAS]
- intracellular transport of virus [TAS]
- ion transmembrane transport [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- negative regulation of type I interferon production [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [TAS]
- positive regulation of NF-kappaB transcription factor activity [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- positive regulation of type I interferon production [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein polyubiquitination [TAS]
- regulation of apoptotic process [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- regulation of type I interferon production [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- transmembrane transport [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral protein processing [TAS]
- virion assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of PDCL3 as a novel chaperone protein involved in the generation of functional VEGF receptor 2.
Angiogenesis, a hallmark step in tumor metastasis and ocular neovascularization, is driven primarily by the function of VEGF ligand on one of its receptors, VEGF receptor 2 (VEGFR-2). Central to the proliferation and ensuing angiogenesis of endothelial cells, the abundance of VEGFR-2 on the surface of endothelial cells is essential for VEGF to recognize and activate VEGFR-2. We have identified ... [more]
J. Biol. Chem. Aug. 09, 2013; 288(32);23171-81 [Pubmed: 23792958]
Throughput
- Low Throughput
Curated By
- BioGRID