An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina.

Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM

In the inner plexiform layer (IPL) of the mouse retina, ~70 neuronal subtypes organize their neurites into an intricate laminar structure that underlies visual processing. To find recognition proteins involved in lamination, we utilized microarray data from 13 subtypes to identify differentially-expressed extracellular proteins and performed a high-throughput biochemical screen. We identified ~50 previously-unknown receptor-ligand pairs, including new interactions among ... [more]

Elife Dec. 04, 2015; 4(0); [Pubmed: 26633812]

Throughput

High Throughput

Additional Notes

ELISA-based HTP assay

Curated By

BioGRID

Interaction Summary

PLXND1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein domain specific binding [ISO]semaphorin receptor activity [IDA, IPI]

Gene Ontology Cellular Component

SEMA4C