BAIT
AKAP6
mAkap
A kinase (PRKA) anchor protein 6
GO Process (21)
GO Function (10)
GO Component (14)
Gene Ontology Biological Process
- cellular protein complex localization [IMP]
- cellular response to cAMP [ISO]
- cellular response to cytokine stimulus [IMP]
- cellular response to epinephrine stimulus [IMP]
- negative regulation of cAMP biosynthetic process [IMP]
- positive regulation of NFAT protein import into nucleus [IMP]
- positive regulation of cAMP-dependent protein kinase activity [IMP]
- positive regulation of calcineurin-NFAT signaling cascade [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell growth involved in cardiac muscle cell development [IMP]
- positive regulation of delayed rectifier potassium channel activity [ISO]
- positive regulation of potassium ion transmembrane transport [ISO]
- positive regulation of protein phosphatase type 2B activity [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of release of sequestered calcium ion into cytosol [IMP]
- positive regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of membrane repolarization [ISO]
- regulation of protein kinase A signaling [IMP]
- regulation of relaxation of cardiac muscle [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [IC]
Gene Ontology Molecular Function- adenylate cyclase binding [IDA]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- protein N-terminus binding [IPI]
- protein anchor [IDA]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein kinase A binding [IDA]
- protein kinase A regulatory subunit binding [IDA, IMP]
- protein phosphatase 2A binding [IDA]
- adenylate cyclase binding [IDA]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- protein N-terminus binding [IPI]
- protein anchor [IDA]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein kinase A binding [IDA]
- protein kinase A regulatory subunit binding [IDA, IMP]
- protein phosphatase 2A binding [IDA]
Gene Ontology Cellular Component
- T-tubule [IDA]
- calcium channel complex [IDA, ISO]
- caveola [IDA]
- cytoplasm [IDA]
- intercalated disc [IDA]
- junctional sarcoplasmic reticulum membrane [IDA]
- nuclear envelope [IDA, ISO]
- nuclear membrane [IDA]
- nuclear outer membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA, ISO]
- protein complex [IDA]
- sarcolemma [IDA]
- sarcoplasmic reticulum [IDA, ISO]
Rattus norvegicus
PREY
RYR2
RYR-2, RyR
ryanodine receptor 2, cardiac
GO Process (42)
GO Function (16)
GO Component (20)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- Purkinje myocyte to ventricular cardiac muscle cell signaling [ISO]
- calcium ion transmembrane transport [IMP, ISO]
- calcium ion transport [ISO, ISS]
- calcium ion transport into cytosol [ISO]
- calcium-mediated signaling [ISO, ISS]
- calcium-mediated signaling using intracellular calcium source [ISO]
- canonical Wnt signaling pathway [ISO]
- cardiac muscle contraction [ISO]
- cardiac muscle hypertrophy [ISO]
- cellular calcium ion homeostasis [ISO, ISS]
- cellular response to caffeine [ISO, ISS]
- cytosolic calcium ion homeostasis [IMP]
- cytosolic calcium ion transport [ISO]
- detection of calcium ion [ISO]
- embryonic heart tube morphogenesis [ISO, ISS]
- establishment of protein localization to endoplasmic reticulum [ISO]
- ion transmembrane transport [IMP]
- left ventricular cardiac muscle tissue morphogenesis [ISO]
- manganese ion transmembrane transport [IMP]
- negative regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of calcium-transporting ATPase activity [ISO]
- positive regulation of heart rate [ISO]
- positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction [ISO]
- positive regulation of sequestering of calcium ion [ISO]
- positive regulation of the force of heart contraction [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [ISO]
- regulation of heart rate [ISO]
- release of sequestered calcium ion into cytosol [ISO]
- release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISO]
- response to caffeine [ISO]
- response to calcium ion [IDA]
- response to drug [IEP]
- response to hypoxia [ISO]
- response to magnesium ion [IDA]
- response to muscle stretch [ISO]
- response to nutrient [IEP]
- response to redox state [ISO]
- sarcoplasmic reticulum calcium ion transport [IMP]
- type B pancreatic cell apoptotic process [ISO]
- ventricular cardiac muscle cell action potential [ISO]
Gene Ontology Molecular Function- calcium channel activity [ISO, ISS]
- calcium-induced calcium release activity [IDA, ISO]
- calcium-release channel activity [ISO]
- calmodulin binding [ISO]
- enzyme binding [ISO]
- identical protein binding [ISO]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- organic cyclic compound binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [ISO]
- protein self-association [ISO]
- ryanodine-sensitive calcium-release channel activity [IDA, IMP, ISO]
- scaffold protein binding [IPI]
- suramin binding [ISO]
- calcium channel activity [ISO, ISS]
- calcium-induced calcium release activity [IDA, ISO]
- calcium-release channel activity [ISO]
- calmodulin binding [ISO]
- enzyme binding [ISO]
- identical protein binding [ISO]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- organic cyclic compound binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [ISO]
- protein self-association [ISO]
- ryanodine-sensitive calcium-release channel activity [IDA, IMP, ISO]
- scaffold protein binding [IPI]
- suramin binding [ISO]
Gene Ontology Cellular Component
- A band [IDA]
- Z disc [IDA, ISO]
- calcium channel complex [ISO]
- cell [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- intracellular [ISO]
- membrane [ISO]
- neuron projection [IDA]
- nuclear envelope [IDA]
- plasma membrane [IDA]
- protein complex [IDA, ISO]
- sarcolemma [IDA]
- sarcomere [ISO]
- sarcoplasmic reticulum [IDA, ISO]
- sarcoplasmic reticulum membrane [IDA, ISO]
- smooth endoplasmic reticulum [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.
The physical association of regulatory enzymes and ion channels at relevant intracellular sites contributes to the diversity and specificity of second messenger-mediated signal transduction in cells. mAKAP is a scaffolding protein that targets the cAMP-dependent protein kinase A and phosphodiesterase type 4D3 to the nuclear envelope of differentiated cardiac myocytes. Here we present data that the mAKAP signaling complex also ... [more]
J. Cell. Sci. Sep. 01, 2001; 114(Pt 17);3167-76 [Pubmed: 11590243]
Throughput
- Low Throughput
Curated By
- BioGRID