BAIT
AKAP6
mAkap
A kinase (PRKA) anchor protein 6
GO Process (21)
GO Function (10)
GO Component (14)
Gene Ontology Biological Process
- cellular protein complex localization [IMP]
- cellular response to cAMP [ISO]
- cellular response to cytokine stimulus [IMP]
- cellular response to epinephrine stimulus [IMP]
- negative regulation of cAMP biosynthetic process [IMP]
- positive regulation of NFAT protein import into nucleus [IMP]
- positive regulation of cAMP-dependent protein kinase activity [IMP]
- positive regulation of calcineurin-NFAT signaling cascade [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell growth involved in cardiac muscle cell development [IMP]
- positive regulation of delayed rectifier potassium channel activity [ISO]
- positive regulation of potassium ion transmembrane transport [ISO]
- positive regulation of protein phosphatase type 2B activity [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of release of sequestered calcium ion into cytosol [IMP]
- positive regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of membrane repolarization [ISO]
- regulation of protein kinase A signaling [IMP]
- regulation of relaxation of cardiac muscle [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [IC]
Gene Ontology Molecular Function- adenylate cyclase binding [IDA]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- protein N-terminus binding [IPI]
- protein anchor [IDA]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein kinase A binding [IDA]
- protein kinase A regulatory subunit binding [IDA, IMP]
- protein phosphatase 2A binding [IDA]
- adenylate cyclase binding [IDA]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- protein N-terminus binding [IPI]
- protein anchor [IDA]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein kinase A binding [IDA]
- protein kinase A regulatory subunit binding [IDA, IMP]
- protein phosphatase 2A binding [IDA]
Gene Ontology Cellular Component
- T-tubule [IDA]
- calcium channel complex [IDA, ISO]
- caveola [IDA]
- cytoplasm [IDA]
- intercalated disc [IDA]
- junctional sarcoplasmic reticulum membrane [IDA]
- nuclear envelope [IDA, ISO]
- nuclear membrane [IDA]
- nuclear outer membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA, ISO]
- protein complex [IDA]
- sarcolemma [IDA]
- sarcoplasmic reticulum [IDA, ISO]
Rattus norvegicus
PREY
PPP2CA
Pp2a1
protein phosphatase 2, catalytic subunit, alpha isozyme
GO Process (26)
GO Function (16)
GO Component (9)
Gene Ontology Biological Process
- cardiac ventricle development [IEP]
- cellular response to calcium ion [IEP]
- cellular response to cytokine stimulus [IEP]
- cellular response to ethanol [IEP]
- cellular response to follicle-stimulating hormone stimulus [IEP]
- cellular response to glucose stimulus [IEP, IMP]
- cellular response to insulin stimulus [IEP]
- cellular response to steroid hormone stimulus [IEP]
- heart development [IEP]
- mesoderm development [ISO]
- negative regulation of calcium ion transmembrane transporter activity [IMP]
- negative regulation of epithelial to mesenchymal transition [ISO]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of transcription factor import into nucleus [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of protein dephosphorylation [IDA]
- positive regulation of protein phosphatase type 2A activity [IMP]
- positive regulation of protein serine/threonine kinase activity [ISO]
- protein dephosphorylation [IDA]
- protein heterotrimerization [IMP]
- regulation of protein autophosphorylation [IMP]
- regulation of protein catabolic process [IMP]
- regulation of protein phosphorylation [IMP]
- regulation of receptor activity [IMP]
Gene Ontology Molecular Function- GABA receptor binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI]
- protein C-terminus binding [IPI, ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein dimerization activity [IMP]
- protein domain specific binding [IDA]
- protein heterodimerization activity [ISS]
- protein kinase B binding [IPI]
- protein kinase binding [IDA]
- protein phosphatase 2A binding [IDA, IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, IMP, TAS]
- tau protein binding [IPI]
- GABA receptor binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI]
- protein C-terminus binding [IPI, ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein dimerization activity [IMP]
- protein domain specific binding [IDA]
- protein heterodimerization activity [ISS]
- protein kinase B binding [IPI]
- protein kinase binding [IDA]
- protein phosphatase 2A binding [IDA, IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, IMP, TAS]
- tau protein binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.
The physical association of regulatory enzymes and ion channels at relevant intracellular sites contributes to the diversity and specificity of second messenger-mediated signal transduction in cells. mAKAP is a scaffolding protein that targets the cAMP-dependent protein kinase A and phosphodiesterase type 4D3 to the nuclear envelope of differentiated cardiac myocytes. Here we present data that the mAKAP signaling complex also ... [more]
J. Cell. Sci. Sep. 01, 2001; 114(Pt 17);3167-76 [Pubmed: 11590243]
Throughput
- Low Throughput
Curated By
- BioGRID