BAIT

PPP2CA

PP2Ac, PP2CA, PP2Calpha, RP-C
protein phosphatase 2, catalytic subunit, alpha isozyme
Homo sapiens
PREY

AKAP6

ADAP100, ADAP6, AKAP100, PRKA6, mAKAP
A kinase (PRKA) anchor protein 6
GO Process (19)
GO Function (7)
GO Component (9)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.

Kapiloff MS, Jackson N, Airhart N

The physical association of regulatory enzymes and ion channels at relevant intracellular sites contributes to the diversity and specificity of second messenger-mediated signal transduction in cells. mAKAP is a scaffolding protein that targets the cAMP-dependent protein kinase A and phosphodiesterase type 4D3 to the nuclear envelope of differentiated cardiac myocytes. Here we present data that the mAKAP signaling complex also ... [more]

J. Cell. Sci. Sep. 01, 2001; 114(Pt 17);3167-76 [Pubmed: 11590243]

Throughput

  • Low Throughput

Curated By

  • BioGRID