BAIT
CAT
catalase
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- UV protection [IMP]
- hydrogen peroxide catabolic process [IDA]
- negative regulation of apoptotic process [IMP]
- nucleobase-containing small molecule metabolic process [TAS]
- osteoblast differentiation [IDA]
- protein homotetramerization [IDA]
- protein tetramerization [IDA]
- purine nucleobase metabolic process [TAS]
- purine nucleotide catabolic process [TAS]
- response to reactive oxygen species [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
G6PD
G6PD1
glucose-6-phosphate dehydrogenase
GO Process (18)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- NADP metabolic process [IDA]
- NADPH regeneration [IMP]
- carbohydrate metabolic process [TAS]
- cellular response to oxidative stress [IMP]
- cholesterol biosynthetic process [IMP]
- cytokine production [IMP]
- erythrocyte maturation [IMP]
- glucose 6-phosphate metabolic process [IDA, IMP]
- glutathione metabolic process [IMP]
- lipid metabolic process [TAS]
- negative regulation of protein glutathionylation [IMP]
- oxidation-reduction process [IMP]
- pentose biosynthetic process [IDA]
- pentose-phosphate shunt [IDA, TAS]
- pentose-phosphate shunt, oxidative branch [IMP]
- ribose phosphate biosynthetic process [IMP]
- small molecule metabolic process [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID