BAIT
CAT
catalase
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- UV protection [IMP]
- hydrogen peroxide catabolic process [IDA]
- negative regulation of apoptotic process [IMP]
- nucleobase-containing small molecule metabolic process [TAS]
- osteoblast differentiation [IDA]
- protein homotetramerization [IDA]
- protein tetramerization [IDA]
- purine nucleobase metabolic process [TAS]
- purine nucleotide catabolic process [TAS]
- response to reactive oxygen species [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CROT
COT
carnitine O-octanoyltransferase
GO Process (9)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- carnitine metabolic process [ISS]
- cellular lipid metabolic process [TAS]
- coenzyme A metabolic process [ISS]
- fatty acid beta-oxidation [IDA]
- fatty acid beta-oxidation using acyl-CoA oxidase [TAS]
- fatty acid metabolic process [IMP]
- generation of precursor metabolites and energy [IDA]
- medium-chain fatty acid metabolic process [IDA]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID