EZR
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- actin filament [IDA]
- apical part of cell [IDA]
- basolateral plasma membrane [ISS]
- cell periphery [IDA]
- cortical cytoskeleton [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of membrane [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- microvillus [IDA]
- nucleolus [IDA]
- plasma membrane [IDA]
- ruffle [IDA]
- vesicle [IDA]
ANK3
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [IMP]
- axonogenesis [ISS]
- cytoskeletal anchoring at plasma membrane [TAS]
- establishment of protein localization [IMP]
- maintenance of protein location in plasma membrane [IGI]
- membrane assembly [IMP]
- mitotic cytokinesis [IMP]
- neuronal action potential [ISS]
- plasma membrane organization [IMP]
- positive regulation of gene expression [ISS]
- positive regulation of membrane depolarization during cardiac muscle cell action potential [ISS]
- positive regulation of membrane potential [ISS]
- positive regulation of sodium ion transmembrane transporter activity [ISS]
- positive regulation of sodium ion transport [ISS]
- protein localization to plasma membrane [IGI, IMP]
- protein targeting to plasma membrane [IMP]
- regulation of potassium ion transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [ISS]
- axon initial segment [ISS]
- basal plasma membrane [IDA]
- basolateral plasma membrane [IDA]
- cell surface [ISS]
- costamere [TAS]
- endoplasmic reticulum [TAS]
- intercalated disc [ISS]
- lateral plasma membrane [IDA]
- node of Ranvier [ISS]
- plasma membrane [ISS]
- sarcolemma [IDA]
- spectrin-associated cytoskeleton [ISS]
- tight junction [IDA]
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
EZR ANK3 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 5.72 | BioGRID | 2988222 |
Curated By
- BioGRID