BAIT
EZR
CVIL, CVL, HEL-S-105, VIL2
ezrin
GO Process (7)
GO Function (4)
GO Component (18)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- actin filament [IDA]
- apical part of cell [IDA]
- basolateral plasma membrane [ISS]
- cell periphery [IDA]
- cortical cytoskeleton [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of membrane [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- microvillus [IDA]
- nucleolus [IDA]
- plasma membrane [IDA]
- ruffle [IDA]
- vesicle [IDA]
Homo sapiens
PREY
PRMT7
protein arginine methyltransferase 7
GO Process (10)
GO Function (9)
GO Component (5)
Gene Ontology Biological Process
- DNA methylation involved in gamete generation [ISS]
- histone arginine methylation [IBA, ISS]
- histone methylation [IDA]
- peptidyl-arginine methylation [IDA, IMP]
- peptidyl-arginine methylation, to asymmetrical-dimethyl arginine [IBA]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IDA, IMP]
- regulation of gene expression by genetic imprinting [ISS]
- regulation of protein binding [IC]
- regulation of transcription, DNA-templated [IBA]
- spliceosomal snRNP assembly [IMP]
Gene Ontology Molecular Function- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- [myelin basic protein]-arginine N-methyltransferase activity [IDA]
- histone binding [IC]
- histone methyltransferase activity (H4-R3 specific) [ISS]
- histone-arginine N-methyltransferase activity [IDA]
- protein-arginine omega-N asymmetric methyltransferase activity [IBA]
- protein-arginine omega-N monomethyltransferase activity [IDA]
- protein-arginine omega-N symmetric methyltransferase activity [IDA, IMP]
- ribonucleoprotein complex binding [IPI]
- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- [myelin basic protein]-arginine N-methyltransferase activity [IDA]
- histone binding [IC]
- histone methyltransferase activity (H4-R3 specific) [ISS]
- histone-arginine N-methyltransferase activity [IDA]
- protein-arginine omega-N asymmetric methyltransferase activity [IBA]
- protein-arginine omega-N monomethyltransferase activity [IDA]
- protein-arginine omega-N symmetric methyltransferase activity [IDA, IMP]
- ribonucleoprotein complex binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID