BAIT
LAMP1
CD107a, LAMPA, LGP120
lysosomal-associated membrane protein 1
GO Process (7)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- establishment of protein localization to organelle [IMP]
- granzyme-mediated apoptotic signaling pathway [IMP]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- protein stabilization [ISS]
- regulation of organelle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RFTN1
PIB10, PIG9, RAFTLIN, MIG2
raftlin, lipid raft linker 1
GO Process (11)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- B cell receptor signaling pathway [IMP]
- T cell antigen processing and presentation [ISS]
- T cell receptor signaling pathway [ISS]
- dsRNA transport [IMP]
- growth [IMP]
- interleukin-17 production [ISS]
- membrane raft assembly [IMP]
- protein localization to membrane raft [ISS]
- protein transport into membrane raft [ISS]
- response to exogenous dsRNA [IMP]
- toll-like receptor 3 signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID