LAMP1
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- establishment of protein localization to organelle [IMP]
- granzyme-mediated apoptotic signaling pathway [IMP]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- protein stabilization [ISS]
- regulation of organelle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLCB1
Gene Ontology Biological Process
- G-protein coupled acetylcholine receptor signaling pathway [ISS]
- G2/M transition of mitotic cell cycle [ISS]
- activation of meiosis involved in egg activation [ISS]
- cerebral cortex development [ISS]
- glutamate receptor signaling pathway [ISS]
- inositol phosphate metabolic process [TAS]
- insulin-like growth factor receptor signaling pathway [ISS]
- interleukin-1-mediated signaling pathway [IDA]
- interleukin-12-mediated signaling pathway [IDA]
- interleukin-15-mediated signaling pathway [IDA]
- memory [ISS]
- negative regulation of monocyte extravasation [ISS]
- negative regulation of transcription, DNA-templated [ISS]
- phosphatidylinositol metabolic process [ISS]
- positive regulation of CD24 biosynthetic process [ISS]
- positive regulation of G1/S transition of mitotic cell cycle [ISS]
- positive regulation of GTPase activity [IDA]
- positive regulation of JNK cascade [IDA]
- positive regulation of acrosome reaction [ISS]
- positive regulation of developmental growth [ISS]
- positive regulation of embryonic development [ISS]
- positive regulation of interleukin-12 production [ISS]
- positive regulation of myoblast differentiation [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of G-protein coupled receptor protein signaling pathway [ISS]
- regulation of fertilization [ISS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LAMP1 PLCB1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 80 | BioGRID | 2992421 |
Curated By
- BioGRID