Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Sorting nexin 9 participates in clathrin-mediated endocytosis through interactions with the core components.

Lundmark R, Carlsson SR

Sorting nexin 9 (SNX9) belongs to a family of proteins, the sorting nexins, that are characterized by the presence of a subclass of the phosphoinositide-binding phox domain. SNX9 has in its amino terminus a Src homology 3 domain and a region with predicted low complexity followed by a carboxyl-terminal part containing the phox domain. We previously found that SNX9 is ... [more]

J. Biol. Chem. Nov. 21, 2003; 278(47);46772-81 [Pubmed: 12952949]

Throughput

  • Low Throughput

Curated By

  • BioGRID