BAIT
BET1
HBET1
Bet1 golgi vesicular membrane trafficking protein
GO Process (0)
GO Function (1)
GO Component (1)
Gene Ontology Molecular Function
Homo sapiens
PREY
GJA1
AVSD3, CMDR, CX43, GJAL, HLHS1, HSS, ODDD
gap junction protein, alpha 1, 43kDa
GO Process (11)
GO Function (4)
GO Component (11)
Gene Ontology Biological Process
- atrial cardiac muscle cell action potential [TAS]
- cell communication by electrical coupling [IDA]
- cell-cell signaling [TAS]
- gap junction assembly [TAS]
- heart development [TAS]
- ion transmembrane transport [TAS]
- membrane organization [TAS]
- muscle contraction [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- signal transduction [IMP]
- transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISS]
- Golgi membrane [TAS]
- Golgi-associated vesicle membrane [TAS]
- endoplasmic reticulum membrane [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- gap junction [IDA, ISS]
- integral component of plasma membrane [TAS]
- intercalated disc [IDA, ISS]
- membrane raft [ISS]
- plasma membrane [ISS, TAS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID