BAIT

BET1

HBET1
Bet1 golgi vesicular membrane trafficking protein
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CKAP4

CLIMP-63, ERGIC-63, p63
cytoskeleton-associated protein 4
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.

Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Oehman T, Varjosalo M

Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]

Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]

Throughput

  • High Throughput

Curated By

  • BioGRID