An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.

Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Oehman T, Varjosalo M

Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]

Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

EEA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

1-phosphatidylinositol binding [IDA]GTP-dependent protein binding [IDA]calmodulin binding [NAS]protein binding [IPI]protein homodimerization activity [IDA]zinc ion binding [TAS]

Gene Ontology Cellular Component

VPS45