EZR
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- actin filament [IDA]
- apical part of cell [IDA]
- basolateral plasma membrane [ISS]
- cell periphery [IDA]
- cortical cytoskeleton [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of membrane [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- microvillus [IDA]
- nucleolus [IDA]
- plasma membrane [IDA]
- ruffle [IDA]
- vesicle [IDA]
RPA3
Gene Ontology Biological Process
- DNA recombinase assembly [TAS]
- DNA repair [TAS]
- DNA replication [IMP, TAS]
- DNA strand elongation involved in DNA replication [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- base-excision repair [IDA]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IMP, TAS]
- mismatch repair [IMP]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [IMP, TAS]
- nucleotide-excision repair, DNA damage removal [TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPA3 EZR | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3636680 |
Curated By
- BioGRID