BAIT

RARA

NR1B1, RAR
retinoic acid receptor, alpha
GO Process (24)
GO Function (15)
GO Component (6)
Homo sapiens
PREY

HDAC3

HD3, RPD3, RPD3-2
histone deacetylase 3
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interaction of nuclear receptor zinc finger DNA binding domains with histone deacetylase.

Franco PJ, Li G, Wei LN

A direct interaction between the nuclear receptor TR2 and histone deacetylases (HDACs) 3 and 4 is mediated by the DNA binding domain (DBD) of TR2. To test if this interaction is common to members of the nuclear receptor family, the Cys2-Cys2 type zinc finger (ZF) DBDs were subcloned from several nuclear receptors (mRARalpha, mRXRbeta, mTR2, mTR4, RAR, mPPARdelta, and mPPARgamma2). ... [more]

Mol. Cell. Endocrinol. Aug. 29, 2003; 206(1);1-12 [Pubmed: 12943985]

Throughput

  • Low Throughput

Curated By

  • BioGRID