Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction of the ERC family of RIM-binding proteins with the liprin-alpha family of multidomain proteins.

Ko J, Na M, Kim S, Lee JR, Kim E

Liprin-alpha/SYD-2 is a family of multidomain proteins with four known isoforms. One of the reported functions of liprin-alpha is to regulate the development of presynaptic active zones, but the underlying mechanism is poorly understood. Here we report that liprin-alpha directly interacts with the ERC (ELKS-Rab6-interacting protein-CAST) family of proteins, members of which are known to bind RIMs, the active zone ... [more]

J. Biol. Chem. Oct. 24, 2003; 278(43);42377-85 [Pubmed: 12923177]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PPFIA4 ERC2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ko J (2003)	Low	-	BioGRID	-
ERC2 PPFIA4	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Ko J (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PPFIA4

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

ERC2