BAIT

BRF1

PCF4, TDS4, transcription factor TFIIIB subunit BRF1, TFIIIB70, L000000193, YGR246C

TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB

GO Process (4)

GO Function (4)

GO Component (1)

Gene Ontology Biological Process

DNA-templated transcriptional open complex formation [IMP]

RNA polymerase III transcriptional preinitiation complex assembly [IDA, IMP]

negative regulation of protein homodimerization activity [IDA]

transposon integration [IDA]

Gene Ontology Molecular Function

DNA binding [IDA]
RNA polymerase III core binding [IDA, IPI]
TFIIIB-type transcription factor activity [IC]
TFIIIC-class transcription factor binding [IDA, IPI]

Gene Ontology Cellular Component

transcription factor TFIIIB complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPC10

RPB12, DNA-directed RNA polymerase core subunit RPB12, ABC10-alpha, L000001686, YHR143W-A

RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress

GO Process (4)

GO Function (5)

GO Component (6)

Gene Ontology Biological Process

tRNA transcription from RNA polymerase III promoter [IDA]

transcription from RNA polymerase I promoter [IDA, IMP]

transcription from RNA polymerase II promoter [IMP]

transcription from RNA polymerase III promoter [IGI, IMP]

Gene Ontology Molecular Function

RNA polymerase I activity [IDA]
RNA polymerase II activity [IDA]
RNA polymerase III activity [IDA]
RNA-directed RNA polymerase activity [IDA]
zinc ion binding [IDA]

Gene Ontology Cellular Component

DNA-directed RNA polymerase I complex [IDA]

DNA-directed RNA polymerase II, core complex [IDA]

DNA-directed RNA polymerase III complex [IDA]

cytoplasm [IDA]

nucleolus [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II.

Bhalla P, Vernekar DV, Gilquin B, Coute Y, Bhargava P

Eukaryotic transcription is a highly regulated fundamental life process. A large number of regulatory proteins and complexes, many of them with sequence-specific DNA-binding activity are known to influence transcription by RNA polymerase (pol) II with a fine precision. In comparison, only a few regulatory proteins are known for pol III, which transcribes genes encoding small, stable, non-translated RNAs. The pol ... [more]

Gene Jun. 20, 2019; 702();205-214 [Pubmed: 30593915]

Throughput

High Throughput

Additional Notes

active state interactome
repressed state interactome

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRF1 RPC10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3611793

Curated By

BioGRID

Interaction Summary

BRF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]RNA polymerase III core binding [IDA, IPI]TFIIIB-type transcription factor activity [IC]TFIIIC-class transcription factor binding [IDA, IPI]

Gene Ontology Cellular Component

RPC10

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase I activity [IDA]RNA polymerase II activity [IDA]RNA polymerase III activity [IDA]RNA-directed RNA polymerase activity [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II.

Throughput

Additional Notes

Related interactions

Curated By

DNA binding [IDA]
RNA polymerase III core binding [IDA, IPI]
TFIIIB-type transcription factor activity [IC]
TFIIIC-class transcription factor binding [IDA, IPI]

RNA polymerase I activity [IDA]
RNA polymerase II activity [IDA]
RNA polymerase III activity [IDA]
RNA-directed RNA polymerase activity [IDA]
zinc ion binding [IDA]