BAIT

ERC2

CAST, CAST1, ELKSL, SPBC110, Spc110
ELKS/RAB6-interacting/CAST family member 2
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

GRIP2

glutamate receptor interacting protein 2
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction of the ERC family of RIM-binding proteins with the liprin-alpha family of multidomain proteins.

Ko J, Na M, Kim S, Lee JR, Kim E

Liprin-alpha/SYD-2 is a family of multidomain proteins with four known isoforms. One of the reported functions of liprin-alpha is to regulate the development of presynaptic active zones, but the underlying mechanism is poorly understood. Here we report that liprin-alpha directly interacts with the ERC (ELKS-Rab6-interacting protein-CAST) family of proteins, members of which are known to bind RIMs, the active zone ... [more]

J. Biol. Chem. Oct. 24, 2003; 278(43);42377-85 [Pubmed: 12923177]

Throughput

  • Low Throughput

Curated By

  • BioGRID