BAIT
LAMP1
CD107a, LAMPA, LGP120
lysosomal-associated membrane protein 1
GO Process (7)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- establishment of protein localization to organelle [IMP]
- granzyme-mediated apoptotic signaling pathway [IMP]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- protein stabilization [ISS]
- regulation of organelle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ADAM10
AD10, AD18, CD156c, HsT18717, MADM, RAK, kuz
ADAM metallopeptidase domain 10
GO Process (20)
GO Function (8)
GO Component (13)
Gene Ontology Biological Process
- Notch receptor processing [TAS]
- Notch signaling pathway [ISS, TAS]
- PMA-inducible membrane protein ectodomain proteolysis [IMP]
- cell-cell signaling [NAS]
- collagen catabolic process [TAS]
- constitutive protein ectodomain proteolysis [IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- in utero embryonic development [ISS]
- integrin-mediated signaling pathway [NAS]
- membrane protein ectodomain proteolysis [IDA, IMP]
- monocyte activation [IMP]
- negative regulation of cell adhesion [IDA, NAS]
- positive regulation of T cell chemotaxis [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP]
- protein phosphorylation [ISS]
- response to tumor necrosis factor [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Golgi-associated vesicle [IDA]
- cell surface [IDA]
- cytoplasm [ISS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- integral component of membrane [NAS]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- nucleus [ISS]
- perinuclear endoplasmic reticulum [IDA]
- plasma membrane [TAS]
- tetraspanin-enriched microdomain [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID