BAIT
LAMP1
CD107a, LAMPA, LGP120
lysosomal-associated membrane protein 1
GO Process (7)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- establishment of protein localization to organelle [IMP]
- granzyme-mediated apoptotic signaling pathway [IMP]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- protein stabilization [ISS]
- regulation of organelle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CHP1
CHP, SLC9A1BP, Sid470p, p22, p24
calcineurin-like EF-hand protein 1
GO Process (24)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- cellular response to acidic pH [ISS]
- cytoplasmic microtubule organization [ISS]
- membrane docking [ISS]
- membrane fusion [ISS]
- membrane organization [ISS]
- microtubule bundle formation [ISS]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of calcineurin-NFAT signaling cascade [IDA]
- negative regulation of phosphatase activity [IDA]
- negative regulation of protein autophosphorylation [ISS]
- negative regulation of protein import into nucleus [IDA]
- negative regulation of protein kinase activity [ISS]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of protein ubiquitination [ISS]
- positive regulation of protein glycosylation [ISS]
- positive regulation of protein targeting to membrane [ISS]
- positive regulation of protein transport [ISS]
- positive regulation of sodium:proton antiporter activity [IDA, ISS]
- potassium ion transport [TAS]
- protein export from nucleus [ISS]
- protein stabilization [ISS]
- regulation of intracellular pH [IDA]
- regulation of neuron death [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID