LMNA
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular protein metabolic process [TAS]
- cellular response to hypoxia [IEP]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment or maintenance of microtubule cytoskeleton polarity [ISS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- mitotic nuclear envelope reassembly [TAS]
- muscle organ development [IMP]
- positive regulation of cell aging [IDA]
- protein localization to nucleus [ISS]
- regulation of cell migration [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TPR
Gene Ontology Biological Process
- MAPK import into nucleus [IMP]
- RNA export from nucleus [IMP]
- RNA import into nucleus [IDA]
- carbohydrate metabolic process [TAS]
- cellular response to heat [IDA]
- cellular response to interferon-alpha [ISS]
- cytokine-mediated signaling pathway [TAS]
- glucose transport [TAS]
- hexose transport [TAS]
- mRNA export from nucleus in response to heat stress [IDA]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of RNA export from nucleus [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of translational initiation [IMP]
- nuclear matrix organization [IMP]
- nuclear pore complex assembly [IMP]
- nuclear pore organization [IMP]
- positive regulation of heterochromatin assembly [IMP]
- positive regulation of intracellular protein transport [IMP]
- positive regulation of mitotic cell cycle spindle assembly checkpoint [IMP]
- positive regulation of protein export from nucleus [ISS]
- positive regulation of protein import into nucleus [IMP]
- protein export from nucleus [IMP]
- protein import into nucleus [IDA, IMP]
- regulation of glucose transport [TAS]
- regulation of mRNA export from nucleus [IMP]
- regulation of mitotic sister chromatid separation [IMP]
- regulation of protein export from nucleus [IMP]
- regulation of protein import into nucleus [IMP]
- regulation of protein stability [IMP]
- regulation of spindle assembly involved in mitosis [IMP]
- response to epidermal growth factor [IDA]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- chromatin binding [IDA]
- dynein complex binding [IDA]
- heat shock protein binding [IDA]
- mRNA binding [IDA]
- mitogen-activated protein kinase binding [IDA]
- nucleocytoplasmic transporter activity [IDA]
- poly(A) RNA binding [IDA]
- protein anchor [IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- transporter activity [IMP]
- tubulin binding [IDA]
- chromatin binding [IDA]
- dynein complex binding [IDA]
- heat shock protein binding [IDA]
- mRNA binding [IDA]
- mitogen-activated protein kinase binding [IDA]
- nucleocytoplasmic transporter activity [IDA]
- poly(A) RNA binding [IDA]
- protein anchor [IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- transporter activity [IMP]
- tubulin binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
LMNA TPR | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3389451 | |
LMNA TPR | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID