LMNA
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular protein metabolic process [TAS]
- cellular response to hypoxia [IEP]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment or maintenance of microtubule cytoskeleton polarity [ISS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- mitotic nuclear envelope reassembly [TAS]
- muscle organ development [IMP]
- positive regulation of cell aging [IDA]
- protein localization to nucleus [ISS]
- regulation of cell migration [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PTPN1
Gene Ontology Biological Process
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- actin cytoskeleton reorganization [IMP]
- blood coagulation [TAS]
- cytokine-mediated signaling pathway [TAS]
- endoplasmic reticulum unfolded protein response [IDA]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of ERK1 and ERK2 cascade [ISS]
- negative regulation of insulin receptor signaling pathway [NAS]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISS]
- peptidyl-tyrosine dephosphorylation [IDA, IMP]
- peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity [ISS]
- platelet activation [TAS]
- platelet-derived growth factor receptor-beta signaling pathway [IMP]
- regulation of endocytosis [IDA]
- regulation of hepatocyte growth factor receptor signaling pathway [IMP]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
- regulation of signal transduction [IMP]
- regulation of type I interferon-mediated signaling pathway [TAS]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
LMNA PTPN1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 16 | BioGRID | 2994599 |
Curated By
- BioGRID