RAB11A
Gene Ontology Biological Process
- GTP catabolic process [IBA, IDA]
- Rab protein signal transduction [IBA]
- astral microtubule organization [IMP]
- cytokinesis [IMP]
- establishment of protein localization to membrane [IMP]
- establishment of protein localization to organelle [IMP]
- establishment of vesicle localization [IMP]
- exocytosis [IBA]
- exosomal secretion [IMP]
- intracellular protein transport [IBA]
- melanosome transport [IBA, ISS]
- mitotic metaphase plate congression [IMP]
- multivesicular body assembly [IMP]
- neuron projection development [IMP]
- plasma membrane to endosome transport [NAS]
- positive regulation of G2/M transition of mitotic cell cycle [IMP]
- positive regulation of epithelial cell migration [IMP]
- protein localization to plasma membrane [IDA]
- regulation of multivesicular body size [IMP]
- regulation of vesicle-mediated transport [IMP]
- spindle assembly involved in mitosis [IMP]
- transmembrane transport [TAS]
- vesicle-mediated transport [IDA]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cleavage furrow [IDA]
- cytoplasmic vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- kinetochore microtubule [IDA]
- multivesicular body [IDA]
- phagocytic vesicle [IDA]
- protein complex [IDA]
- recycling endosome [IBA, IDA, ISS]
- spindle pole [IDA]
- trans-Golgi network [IDA]
- vesicle [IDA]
RALGAPA1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAB11A RALGAPA1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 231 | BioGRID | 3001062 |
Curated By
- BioGRID