LPAR1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
APPL1
Gene Ontology Biological Process
- apoptotic process [TAS]
- cell proliferation [IDA]
- insulin receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- regulation of apoptotic process [TAS]
- regulation of establishment of protein localization to plasma membrane [IMP]
- regulation of glucose import [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The PDZ protein GIPC regulates trafficking of the LPA1 receptor from APPL signaling endosomes and attenuates the cell's response to LPA.
Lysophosphatidic acid (LPA) mediates diverse cellular responses through the activation of at least six LPA receptors--LPA(1-6,) but the interacting proteins and signaling pathways that mediate the specificity of these receptors are largely unknown. We noticed that LPA(1) contains a PDZ binding motif (SVV) identical to that present in two other proteins that interact with the PDZ protein GIPC. GIPC is ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID