BAIT
TGOLN2
TGN38, TGN46, TGN48, TGN51, TTGN2
trans-golgi network protein 2
GO Process (0)
GO Function (1)
GO Component (4)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PICALM
CALM, CLTH, LAP
phosphatidylinositol binding clathrin assembly protein
GO Process (22)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- cargo loading into vesicle [IMP]
- cell proliferation [IMP]
- clathrin coat assembly [IMP]
- clathrin-mediated endocytosis [IMP]
- endosomal transport [IMP]
- iron ion homeostasis [IMP]
- iron ion import into cell [IMP]
- negative regulation of gene expression [IMP]
- negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process [ISS]
- negative regulation of receptor-mediated endocytosis [IDA]
- positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process [ISS]
- positive regulation of beta-amyloid formation [IMP]
- positive regulation of neuron death [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein complex assembly [TAS]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IDA, ISS]
- regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process [IMP]
- regulation of endocytosis [IMP]
- regulation of protein localization [IDA]
- synaptic vesicle maturation [ISS]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- AP-2 adaptor complex [IDA]
- clathrin coat of coated pit [IDA]
- coated pit [IDA, ISS]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- neurofibrillary tangle [IMP]
- neuronal cell body [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISS]
- postsynaptic membrane [ISS]
- presynaptic membrane [ISS]
- vesicle [ISS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID