BAIT

TNPO2

IPO3, KPNB2B, TRN2
transportin 2
GO Process (0)
GO Function (2)
GO Component (0)
Homo sapiens
PREY

A1CF

ACF, ACF64, ACF65, APOBEC1CF, ASP, RP11-564C4.2
APOBEC1 complementation factor
GO Process (4)
GO Function (4)
GO Component (3)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling.

Blanc V, Kennedy S, Davidson NO

C to U editing of the nuclear apolipoprotein B (apoB) transcript is mediated by a core enzyme containing a catalytic deaminase, apobec-1, and an RNA binding subunit, apobec-1 complementation factor (ACF). ACF expression is predominantly nuclear, including mutant proteins with deletions of a putative nuclear localization signal. We have now identified a novel 41-residue motif (ANS) in the auxiliary domain ... [more]

J. Biol. Chem. Oct. 17, 2003; 278(42);41198-204 [Pubmed: 12896982]

Throughput

  • Low Throughput

Curated By

  • BioGRID