BAIT
TGOLN2
TGN38, TGN46, TGN48, TGN51, TTGN2
trans-golgi network protein 2
GO Process (0)
GO Function (1)
GO Component (4)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KIF16B
C20orf23, KISC20ORF, SNX23, RP5-971B4.1
kinesin family member 16B
GO Process (10)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- Golgi to endosome transport [ISS]
- early endosome to late endosome transport [IMP]
- endoderm development [ISS]
- epidermal growth factor receptor signaling pathway [IMP]
- fibroblast growth factor receptor signaling pathway [ISS]
- formation of primary germ layer [ISS]
- microtubule-based movement [IBA]
- receptor catabolic process [IMP]
- regulation of receptor recycling [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID