BAIT
TGOLN2
TGN38, TGN46, TGN48, TGN51, TTGN2
trans-golgi network protein 2
GO Process (0)
GO Function (1)
GO Component (4)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GOLPH3
GOPP1, GPP34, MIDAS, Vps74
golgi phosphoprotein 3 (coat-protein)
GO Process (15)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- Golgi organization [IMP]
- Golgi to plasma membrane protein transport [IMP]
- Golgi vesicle budding [IMP]
- cell adhesion molecule production [IMP]
- cell migration [IMP]
- cell proliferation [IMP]
- gene expression [IMP]
- glycoprotein biosynthetic process [IMP]
- lamellipodium assembly [IMP]
- leukocyte tethering or rolling [IMP]
- positive regulation of TOR signaling [IMP]
- positive regulation of protein secretion [IMP]
- protein retention in Golgi apparatus [IMP]
- protein secretion [IMP]
- regulation of mitochondrion organization [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID