TUBA1A
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DPM1
Gene Ontology Biological Process
- C-terminal protein lipidation [TAS]
- GPI anchor biosynthetic process [IDA]
- cellular protein metabolic process [TAS]
- dolichol metabolic process [IDA]
- dolichol-linked oligosaccharide biosynthetic process [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein O-linked mannosylation [IDA]
- protein mannosylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
TUBA1A DPM1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID