BAIT
LAMP1
CD107a, LAMPA, LGP120
lysosomal-associated membrane protein 1
GO Process (7)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- establishment of protein localization to organelle [IMP]
- granzyme-mediated apoptotic signaling pathway [IMP]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- protein stabilization [ISS]
- regulation of organelle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB13
GIG4
RAB13, member RAS oncogene family
GO Process (15)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISS]
- cortical actin cytoskeleton organization [IBA, ISS]
- endocytic recycling [IMP]
- endosomal transport [IMP]
- endothelial cell chemotaxis [ISS]
- establishment of Sertoli cell barrier [ISS]
- establishment of protein localization to plasma membrane [IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- neuron projection development [IMP]
- protein kinase A signaling [IMP]
- tight junction assembly [IMP]
- trans-Golgi network to recycling endosome transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- endocytic vesicle [IDA]
- extracellular vesicular exosome [IDA]
- insulin-responsive compartment [ISS]
- lamellipodium [ISS]
- lateral plasma membrane [IDA]
- neuron projection [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tight junction [IDA]
- trans-Golgi network [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID