BAIT
EEA1
MST105, MSTP105, ZFYVE2
early endosome antigen 1
GO Process (4)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GTPBP4
CRFG, NGB, NOG1, RP11-38M7.5
GTP binding protein 4
GO Process (10)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- negative regulation of DNA replication [IMP]
- negative regulation of cell migration [IDA]
- negative regulation of cell proliferation [IMP]
- negative regulation of cell-cell adhesion [IDA]
- negative regulation of collagen binding [IMP]
- negative regulation of protein ubiquitination [IDA]
- osteoblast differentiation [IDA]
- protein stabilization [IDA]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID