BAIT
EZR
CVIL, CVL, HEL-S-105, VIL2
ezrin
GO Process (7)
GO Function (4)
GO Component (18)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- actin filament [IDA]
- apical part of cell [IDA]
- basolateral plasma membrane [ISS]
- cell periphery [IDA]
- cortical cytoskeleton [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of membrane [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- microvillus [IDA]
- nucleolus [IDA]
- plasma membrane [IDA]
- ruffle [IDA]
- vesicle [IDA]
Homo sapiens
PREY
BCL11A
BCL11A-L, BCL11A-S, BCL11A-XL, BCL11a-M, CTIP1, EVI9, HBFQTL5, ZNF856
B-cell CLL/lymphoma 11A (zinc finger protein)
GO Process (11)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- negative regulation of axon extension [ISS]
- negative regulation of collateral sprouting [IMP]
- negative regulation of dendrite development [IMP]
- negative regulation of neuron projection development [IDA]
- negative regulation of protein homooligomerization [IC]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of collateral sprouting [IMP]
- positive regulation of neuron projection development [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- protein sumoylation [ISS]
- regulation of dendrite development [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [TAS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID