BAIT
MRM1
mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae)
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
PDK3
CMTX6, GS1-358P8.4
pyruvate dehydrogenase kinase, isozyme 3
GO Process (11)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- cellular metabolic process [TAS]
- cellular response to fatty acid [IMP]
- cellular response to glucose stimulus [ISS]
- hypoxia-inducible factor-1alpha signaling pathway [IMP]
- peptidyl-serine phosphorylation [IDA]
- peroxisome proliferator activated receptor signaling pathway [IMP]
- pyruvate metabolic process [TAS]
- regulation of acetyl-CoA biosynthetic process from pyruvate [IMP, TAS]
- regulation of glucose metabolic process [IMP]
- regulation of reactive oxygen species metabolic process [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID