VDAC2
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BAK1
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity [IDA]
- apoptotic process [TAS]
- apoptotic signaling pathway [IMP]
- cellular response to UV [IMP, ISS]
- cellular response to mechanical stimulus [IEP]
- endoplasmic reticulum calcium ion homeostasis [TAS]
- establishment or maintenance of transmembrane electrochemical gradient [IDA]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA]
- intrinsic apoptotic signaling pathway [TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IBA]
- positive regulation of apoptotic process [IMP]
- positive regulation of endoplasmic reticulum unfolded protein response [IMP]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [TAS]
- positive regulation of proteolysis [IDA]
- regulation of mitochondrial membrane permeability [IDA]
- regulation of mitochondrial membrane potential [IDA]
- regulation of protein heterodimerization activity [IDA]
- regulation of protein homodimerization activity [IDA]
- release of cytochrome c from mitochondria [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
VDAC2 inhibits BAK activation and mitochondrial apoptosis.
The multidomain proapoptotic molecules BAK or BAX are required to initiate the mitochondrial pathway of apoptosis. How cells maintain the potentially lethal proapoptotic effector BAK in a monomeric inactive conformation at mitochondria is unknown. In viable cells, we found BAK complexed with mitochondrial outer-membrane protein VDAC2, a VDAC isoform present in low abundance that interacts specifically with the inactive conformer ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BAK1 VDAC2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.372 | BioGRID | 242331 |
Curated By
- BioGRID